TRPM3 Polyclonal antibody

TRPM3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12724-1-AP

Synonyms

GON 2, LTRPC3, MLSN2, TRPM3

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Validation Data Gallery

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  WB analysis using 12724-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12724-1-AP (TRPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12724-1-AP targets TRPM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPM3 fusion protein Ag3421
• Full Name: transient receptor potential cation channel, subfamily M, member 3
• Calculated Molecular Weight: 1732 aa, 197 kDa
• Observed Molecular Weight: 160-210 kDa
• GenBank Accession Number: BC022454
• Gene Symbol: TRPM3
• Gene ID (NCBI): 80036
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transient receptor potential melastatin-3 (TRPM3) channel belongs to the family of transient receptor potential (TRP) cation channels that are expressed in a variety of tissues and cell types, including kidney, brain, dorsal root ganglia, cardiomyocytes and pancreatic beta-cells. TRPM3 channels can be activated by the neurosteroid pregnenolone sulfate, and they are regulated by phosphoinositides, and perhaps by calmodulin. Stimulation of TRPM3 channels is connected with heat sensation by somatosensory neurons, insulin secretion by pancreatic beta-cells, regulation of neurotransmitter release, iris constriction, and tumor promotion. The calculated MW of TRPM3 is 197 kDa, 12724-1-AP can detect a band around 160 kDa, which likely corresponds to different splice variant. (PMID: 28720517,21198543)