Tested Applications
| Positive WB detected in | 37°C incubated HEK-293 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21985-1-AP targets TRPM4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17132 Product name: Recombinant human TRPM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC132727 Sequence: MVVPEKEQSWIPKIFKKKTCTTFIVDSTDPGGTLCQCGRPRTAHPAVAMEDAFGAAVVTVWDSDAHTTEKPTDAYGELDFTGAGRKHSNFLRLSDRTDPAAVYSLVTRTWGFRAPNLVVSVLGGSGGPVLQTWLQDLLRRGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQMASTGGTKVVAMGVAPWGVVRNRDTLINPKGSFPARYRWRGDPEDGVQFPLDYNYSAFFLVDDGTHGCLGGENRFRLRLESYISQQKTGVGGTGIDIPVLLLLIDGDEKMLTRIENATQAQLPCLLVAGSGGAADCLAETLEDTLAPGSGGARQGEARDRIRRFFPKGDLEVLQAQ Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily M, member 4 |
| Calculated Molecular Weight | 1214 aa, 134 kDa |
| Observed Molecular Weight | 130~150 kDa |
| GenBank Accession Number | BC132727 |
| Gene Symbol | TRPM4 |
| Gene ID (NCBI) | 54795 |
| RRID | AB_2935456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD43 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRPM4 is a Calcium-activated nonselective cation channel. It is activated by increase in intracellular Ca2+, mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. TRPM4 participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. It's also involved in myogenic constriction of cerebral arteries.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRPM4 antibody 21985-1-AP | Download protocol |
| WB protocol for TRPM4 antibody 21985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRPM4 Polyclonal antibody (Cat# 21985-1-AP) has 9 total citations. These appear in journals including Phytomedicine, Frontiers in Immunology, CNS Neuroscience & Therapeutics, Communications Biology, Experimental Neurology, Computational and Structural Biotechnology Journal, Immunotargets and Therapy, Tissue and Cell, and Discovery Oncology. The associated research fields span oncology (breast, prostate, pan-cancer), neuroscience (traumatic brain injury, neuroinflammation), cardiovascular disease (Brugada syndrome, endothelial dysfunction), and immunology, with applications including IHC, IF, and WB.
| Species | Application | Title |
|---|---|---|
Phytomedicine Cinobufagin overcomes bortezomib resistance in multiple myeloma strains by targeting SEC62/TRPM4-mediated NECSO | ||
Front Immunol Necrosis by sodium overload-associated genes TRPM4 and SLC9A1: biological roles and clinical implications in breast cancer progression. | ||
CNS Neurosci Ther Inhibition of Transglutaminase 2 Preserves Blood-Brain Barrier Integrity and Improves Neurological Outcomes After Experimental Traumatic Brain Injury in Mice. | ||
Commun Biol N-palmitoyl glycine differentially modulates TRPM4 and TRPC5 and is causally linked to Brugada syndrome. | ||
Comput Struct Biotechnol J Identification of a 24-gene panel and a novel marker of PODXL2 essential for the pathological diagnosis of early prostate cancer | ||
Exp Neurol Inhibition of the TRPM4 channel by 9-PH attenuates hippocampal endothelial injury induced by sleep deprivation and the subsequent neuroinflammation and neurological dysfunction. |
