Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55251-1-AP targets TRPM7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily M, member 7 |
| Calculated Molecular Weight | 213 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | NM_017672 |
| Gene Symbol | TRPM7 |
| Gene ID (NCBI) | 54822 |
| RRID | AB_2935541 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96QT4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transient receptor potential melastatin 7 (TRPM7), along with the closely related TRPM6, are unique channels that have dual operations: cation permeability and kinase activity. In contrast to the limited tissue distribution of TRPM6, TRPM7 is widely expressed among tissues and is therefore implicated in a variety of cellular functions physiologically and pathophysiologically. The discovery of TRPM7's unique structure imparting dual ion channel and kinase activities shed light onto novel and peculiar biological functions, such as Mg2+ homeostasis, cellular Ca2+ flickering, and even intranuclear transcriptional regulation by a cleaved kinase domain translocated to nuclei. The calculated MW of TRPM7 is 213 kDa, 55251-1-AP can detect a band around 220 kDa. (PMID: 32503408)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPM7 antibody 55251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRPM7 antibody (Cat# 55251-1-AP) has 7 citations across journals including Nature Communications, Advanced Science, International Journal of Biological Sciences, Aging Cell, European Journal of Pharmaceutical Sciences, and Tissue Cell. Research fields span tracheal defect reconstruction, mitochondrial pyroptosis in dental stem cells, lung adenocarcinoma chromatin remodeling, triple-negative breast cancer angiogenesis, vascular senescence, fentanyl-induced analgesia, and testicular ischemia-reperfusion injury. Applications include WB, IHC, and IF in human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Nat Commun Mechanically-driven expandable patch promotes tracheal defect reconstruction via synergistic microcurrent and mechanical force cascades. | ||
Adv Sci (Weinh) Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary-Patterned Malignancy in Lung Adenocarcinoma | ||
Adv Sci (Weinh) LPS-Induced Mitochondrial Damage via SLC41A1-Mediated Magnesium Ion Efflux Leads to the Pyroptosis of Dental Stem Cells | ||
Int J Biol Sci The TRPM7 inhibitor carvacrol suppresses angiogenesis and vasculogenic mimicry in triple-negative breast cancer. | ||
Aging Cell TRPM7 Deficiency Accelerates Vascular Senescence by Inhibiting H3K18 Lactylation.
| ||
Eur J Pharm Sci Fentanyl induces analgesic effect through miR-381-3p/TRPM7 when combined with bupivacaine in subarachnoid injection |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer reported that everything about the antibody performed perfectly, with one minor note that the membrane boxes they had requested were not included in the shipment. Overall, the antibody itself received a fully positive assessment with no issues raised regarding its performance or quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alexis (Verified Customer) (06-11-2026) | Tout est parfait, nous n'avons cependant pas reçu les boites de membrane que nous avions demandé de recevoir
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