Tested Applications
| Positive IHC detected in | human liver cancer tissue, human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12813-1-AP targets TRPM8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3483 Product name: Recombinant human TRPM8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-192 aa of BC001135 Sequence: SFLPVHTIVLIRENVCKCGYAQSQHMEGTQINQSEKWNYKKHTKEFPTDAFGDIQFETLGKKGKYIRLSCDTDAEILYELLTQHWHLKTPNLVISVTGGAKNFALKPRMRKIFSRLIYIAQSKGAWILTGGTHYGLMKYIGEVVRDNTISRSSEENIVAIGIAAWGMVSNRDTLIRNCDAEVPVGQEEVC Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily M, member 8 |
| Calculated Molecular Weight | 1104 aa, 128 kDa |
| GenBank Accession Number | BC001135 |
| Gene Symbol | TRPM8 |
| Gene ID (NCBI) | 79054 |
| RRID | AB_2877883 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z2W7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRPM8, also known as CMR1 (cold-menthol receptor-1), is a nonselective cation channel activated by cold and the cooling substances menthol and icilin (PMID: 11893340; 11882888). It serves as a neuronal sensor of cold temperatures. Functioning TRPM8 channels are required for behavioral responses to innocuous cool, noxious cold, injury-evoked cold hypersensitivity, and cooling-mediated analgesia (PMID: 17538622; 21438154; 16920620; 21984952). Additionally, TRPM8 has also been reported to be involved in thermoregulation and the regulation of basal tear flow (PMID: 21407809; 21076394). TRPM8 is expressed extensively in sensory nerves, human prostate and overexpressed in a variety of cancers including prostate, breast, lung, colon and skin melanomas (PMID: 11325849; 20816009).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TRPM8 antibody 12813-1-AP | Download protocol |
| IF protocol for TRPM8 antibody 12813-1-AP | Download protocol |
| IHC protocol for TRPM8 antibody 12813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRPM8 Polyclonal antibody (Cat# 12813-1-AP) has 8 citations across journals including Biomaterials, Current Research in Food Science, Cancer Gene Therapy, Communications Biology, Inflammation, Journal of Dermatological Science, SLAS Discovery, and Reproduction Biology. Research fields span immunometabolism and wound healing, cooling sensation biosensing, hepatocellular carcinoma, inflammatory pain, asthma, rosacea pruritus, calcium channel assays, and canine testicular tumorigenesis. Applications include IF, WB, and IHC across mouse, human, and canine species.
| Species | Application | Title |
|---|---|---|
Biomaterials Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation. | ||
Curr Res Food Sci Cooling sensation biosensor coupled with the Chou-Talalay method for quantitative assessment of interaction patterns in binary cooling agent mixtures. | ||
Cancer Gene Ther TRPM8 promotes hepatocellular carcinoma progression by inducing SNORA55 mediated nuclear-mitochondrial communication | ||
Commun Biol IL-33/ST2 drives inflammatory pain via CCL2 signaling and activation of TRPV1 and TRPM8 | ||
Inflammation Cold Stimuli Facilitate Inflammatory Responses Through Transient Receptor Potential Melastatin 8 (TRPM8) in Primary Airway Epithelial Cells of Asthmatic Mice. | ||
J Dermatol Sci The temperature-sensitive receptors TRPV4 and TRPM8 have important roles in the pruritus of rosacea |
