TRPS1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83062-4-RR (same clone as 83062-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83062-4-RR (TRPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83062-4-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 83062-4-RR (same clone as 83062-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83062-4-RR (TRPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83062-4-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83062-4-RR (same clone as 83062-4-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TRPS1 Recombinant antibody (83062-4-RR, Clone:230051F10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83062-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83062-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83062-4-RR against Human TRPS1 were performed. The affinity constant is 88.0 pM.

TRPS1 Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 83062-4-PBS

Clone No.230051F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79, 230051F10

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Product Information

83062-4-PBS targets TRPS1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33959

  Product name: Recombinant human TRPS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 9-358 aa of BC125020

  Sequence: LRNVASEGEGQILEPIGTESKVSGKNKEFSADQMSENTDQSDAAELNHKEEHSLHVQDPSSSSKKDLKSAVLSEKAGFNYESPSKGGNFPSFPHDEVTDRNMLAFSSPAAGGVCEPLKSPQRAEADDPQDMACTPSGDSLETKEDQKMSPKATEETGQAQSGQANCQGLSPVSVASKNPQVPSDGGVRLNKSKTDLLVNDNPDPAPLSPELQDFKCNICGYGYYGNDPTDLIKHFRKYHLGLHNRTRQDAELDSKILALHNMVQFSHSKDFQKVNRSVFSGVLQDINSSRPVLLNGTYDVQVTSGGTFIGIGRKTPDCQGNTKYFRCKFCNFTYMGNSSTELEQHFLQTH

• Full Name: trichorhinophalangeal syndrome I
• Calculated Molecular Weight: 1294 aa, 143 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC125020
• Gene Symbol: TRPS1
• Gene ID (NCBI): 7227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UHF7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains.