Anti-Human TRPV4 Rabbit Recombinant Antibody

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• Anti-Human TRPV4 Rabbit Recombinant Antibody (98690-1-RR)

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  SEC-HPLC of 98690-1-RR

  The purity of Human TRPV4 Recombinant monoclonal antibody (Cat. No. 98688-2-RR) was greater than 95% as determined by SEC-HPLC.

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  IHC staining of human colon using 98690-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98690-1-RR (TRPV4 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 98690-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 98690-1-RR (TRPV4 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of THP-1 using 98690-1-RR

  1x10^6 THP-1 cells were surface stained with 0.25 ug Anti-Human TRPV4 Rabbit RecAb (98690-1-RR, Clone:251361G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

TRPV4 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98690-1-RR

Clone No.251361G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

Osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OTRPC4, TRP12, Vanilloid receptor-like channel 2

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: THP-1 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98690-1-RR targets TRPV4 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: transient receptor potential cation channel, subfamily V, member 4
• Calculated Molecular Weight: 98 kDa
• GenBank Accession Number: BC117426
• Gene Symbol: TRPV4
• Gene ID (NCBI): 59341
• RRID: AB_3746278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HBA0
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.