TRUB1 Polyclonal antibody

TRUB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12520-1-AP

Synonyms

PUS4, TRUB1

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Validation Data Gallery

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  WB analysis using 12520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12520-1-AP (TRUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12520-1-AP

  IP result of anti-TRUB1 (IP:12520-1-AP, 4ug; Detection:12520-1-AP 1:300) with mouse liver tissue lysate 6400ug.

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  IHC staining of human kidney using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

12520-1-AP targets TRUB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRUB1 fusion protein Ag3213
• Full Name: TruB pseudouridine (psi) synthase homolog 1 (E. coli)
• Calculated Molecular Weight: 349 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC030601
• Gene Symbol: TRUB1
• Gene ID (NCBI): 142940
• RRID: AB_10640437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.

Publications

Species	Application	Title
human	WB	Nat Methods; Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome; Authors - Haiqi Xu
human	IF	bioRxiv; Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity; Authors - Oleksandra Fanari
human	WB	Nucleic Acids Res; A bisulfite-assisted and ligation-based qPCR amplification technology for locus-specific pseudouridine detection at base resolution; Authors - Xin Fang ; KD Validated