TRUB1 Polyclonal antibody

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  WB analysis using 12520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12520-1-AP (TRUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12520-1-AP

  IP result of anti-TRUB1 (IP:12520-1-AP, 4ug; Detection:12520-1-AP 1:300) with mouse liver tissue lysate 6400ug.

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  IHC staining of human kidney using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12520-1-AP (TRUB1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12520-1-AP (TRUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRUB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12520-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

EC:5.4.99.-, EC:5.4.99.25, Pseudouridylate synthase TRUB1, Psi55 synthase TRUB1, PUS4

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

12520-1-AP targets TRUB1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3213

  Product name: Recombinant human TRUB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-349 aa of BC030601

  Sequence: MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAALATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC

• Full Name: TruB pseudouridine (psi) synthase homolog 1 (E. coli)
• Calculated Molecular Weight: 349 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC030601
• Gene Symbol: TRUB1
• Gene ID (NCBI): 142940
• RRID: AB_10640437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWH5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Methods; Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome; Authors - Haiqi Xu
human	IF	Cell Syst; Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess epitranscriptome plasticity in a neuronal cell line; Authors - Oleksandra Fanari
human	WB	Cancer Discov; ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translation; Authors - Chao Shen
human	IF	bioRxiv; Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity; Authors - Oleksandra Fanari
human	WB	Nucleic Acids Res; A bisulfite-assisted and ligation-based qPCR amplification technology for locus-specific pseudouridine detection at base resolution; Authors - Xin Fang ; KD Validated