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TRUB2 Polyclonal antibody

TRUB2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19891-1-AP

Synonyms

CLONE24922, RP11 339B21.1, TRUB2



Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19891-1-AP targets TRUB2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRUB2 fusion protein Ag13726
Full Name TruB pseudouridine (psi) synthase homolog 2 (E. coli)
Calculated Molecular Weight 331 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001457
Gene Symbol TRUB2
Gene ID (NCBI) 26995
RRIDAB_10640900
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRUB2 antibody 19891-1-APDownload protocol
IHC protocol for TRUB2 antibody 19891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Authors - Hana Antonicka
humanWB

Nat Cell Biol

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression

Authors - Nicholas J Kramer