TSC22D2 Polyclonal antibody

TSC22D2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25418-1-AP

Synonyms

TSC22 domain family protein 2, TILZ4c, TILZ4b, TILZ4a, TILZ4

---

Validation Data Gallery

• 

  WB analysis using 25418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of MDA-MB-453s using 25418-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25418-1-AP (TSC22D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 25418-1-AP

  IP result of anti-TSC22D2 (IP:25418-1-AP, 5ug; Detection:25418-1-AP 1:300) with HepG2 cells lysate 2500ug.

• 

  IHC staining of human kidney using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25418-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25418-1-AP (TSC22D2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 25418-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TSC22D2 antibody (25418-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, MDA-MB-453s cells, HepG2 cells, SH-SY5Y cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human kidney tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

25418-1-AP targets TSC22D2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSC22D2 fusion protein Ag22294
• Full Name: TSC22 domain family, member 2
• Calculated Molecular Weight: 780 aa, 79 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC044643
• Gene Symbol: TSC22D2
• Gene ID (NCBI): 9819
• RRID: AB_2880069
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC22D2 (transforming growth factor β-stimulated clone 22 domain family, member 2) is a member of the TSC-22 domain family comprising putative transcription factors that are characterized by a carboxy-terminal leucine zipper and an adjacent TSC-box. TSC22D2 is expressed at low levels in CRC and that TSC22D2 overexpression significantly inhibited the growth rate of cancer cells (PMID: 27573352).

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer; A novel cuproptosis-related gene model predicts outcomes and treatment responses in pancreatic adenocarcinoma; Authors - Qixian Liu