GILZ Polyclonal antibody

GILZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12352-1-AP

Synonyms

TSC22D3, TSC22 D3, TSC 22R, DSIP-immunoreactive peptide, DSIPI

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Validation Data Gallery

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  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12352-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 12352-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12352-1-AP (GILZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 12352-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using GILZ antibody (12352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12352-1-AP targets GILZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GILZ fusion protein Ag3015
• Full Name: TSC22 domain family, member 3
• Calculated Molecular Weight: 134 aa, 15 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC018148
• Gene Symbol: GILZ
• Gene ID (NCBI): 1831
• RRID: AB_2272196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684).

Publications

Species	Application	Title
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats.; Authors - Ruiping Gu
rat	WB	Invest Ophthalmol Vis Sci; Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells.; Authors - Ruiping Gu
human	WB	Exp Eye Res; Glucocorticoid-induced leucine zipper overexpression inhibits lipopolysaccharide-induced retinal inflammation in rats.; Authors - Ruiping Gu