Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12352-1-AP targets GILZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3015 Product name: Recombinant human TSC22D3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-92 aa of BC018148 Sequence: SGASVVAIDNKIEQAMDLVKNHLMYAVREEVEILKEQIRELVEKNSQLERENTLLKTLASPEQLEKFQSCLSPEEPAPESPQVPEAPGGSAV Predict reactive species |
| Full Name | TSC22 domain family, member 3 |
| Calculated Molecular Weight | 134 aa, 15 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC018148 |
| Gene Symbol | GILZ |
| Gene ID (NCBI) | 1831 |
| RRID | AB_2272196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GILZ antibody 12352-1-AP | Download protocol |
| IHC protocol for GILZ antibody 12352-1-AP | Download protocol |
| WB protocol for GILZ antibody 12352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GILZ Polyclonal Antibody (Cat# 12352-1-AP) has 7 citations published in J Adv Res, Biomaterials, Invest Ophthalmol Vis Sci, Stem Cells Int, Immun Inflamm Dis, and Exp Eye Res. Research fields span cancer gene therapy, calcific aortic valve disease, retinal inflammation and degeneration, osteogenesis of bone marrow stromal cells, and glucocorticoid-mediated immune regulation. Applications cited include WB and IF.
| Species | Application | Title |
|---|---|---|
Biomaterials Modeling calcific aortic valve disease with engineered human valve tissues identifies SAMHD1 as a therapeutic target. | ||
Invest Ophthalmol Vis Sci Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells. | ||
Invest Ophthalmol Vis Sci Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats. | ||
Stem Cells Int Identification and Validation of a New Functional Gene TSC22D3 for hBMSCs Osteogenesis.
| ||
Immun Inflamm Dis Electroacupuncture at ST36/SP6 Restores Surgical Stress-Induced Impairment of Dendritic Cell Maturation via GR/GILZ Signaling in Mice |
