TSEN34 Polyclonal antibody

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  WB analysis using 31212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 31212-1-AP

  WB result of TSEN34 antibody (31212-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSEN34 transfected MCF-7 cells.

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  IF Staining of MCF-7 using 31212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (31212-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

TSEN34 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31212-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:4.6.1.16, HsSen34, LENG5, PCH2C, SEN34

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293 cells, MCF-7 cells, HeLa cells, HEK-293T cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31212-1-AP targets TSEN34 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSEN34 fusion protein Ag35120
• Full Name: tRNA splicing endonuclease 34 homolog (S. cerevisiae)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-36 kDa
• GenBank Accession Number: BC020805
• Gene Symbol: TSEN34
• Gene ID (NCBI): 79042
• RRID: AB_3669904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9BSV6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)