TSEPA Polyclonal antibody

TSEPA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27415-1-AP

Synonyms

C6orf190, C6orf207, Protein THEMIS, THEMIS, TSEPA

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 27415-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27415-1-AP (TSEPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27415-1-AP (TSEPA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27415-1-AP targets TSEPA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSEPA fusion protein Ag26387
• Full Name: thymocyte selection pathway associated
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC130516
• Gene Symbol: TSEPA
• Gene ID (NCBI): 387357
• RRID: AB_2880866
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.