THEMIS Polyclonal antibody

THEMIS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27415-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

TSEPA, Thymocyte-expressed molecule involved in selection, Protein THEMIS, C6orf207, C6orf190

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

27415-1-AP targets THEMIS in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26387

Product name: Recombinant human TSEPA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-222 aa of BC130516

Sequence: AEICEQIEGCESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNSVEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWDSTNPFPKDFYGT

Predict reactive species
Full Name thymocyte selection pathway associated
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC130516
Gene Symbol THEMIS
Gene ID (NCBI) 387357
RRIDAB_2880866
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1K5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The T cell lineage-restricted protein THEMIS, also called C6orf190 and C6orf207, plays a critical role in T cell development at the positive selection stage (PMID: 37159521). THEMIS plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. It is required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells and regulates T-cell development through T-cell antigen receptor (TCR) signaling (PMID: 28697966, 28250424). It can also promote the proliferative response of CD8+ T cells to low-affinity ligands (PMID: 31932808).

Protocols

Product Specific Protocols
WB protocol for THEMIS antibody 27415-1-APDownload protocol
IHC protocol for THEMIS antibody 27415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Theranostics

Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma

Authors - Yao Li
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