TSG101 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84334-4-RR (same clone as 84334-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84334-4-RR (TSG101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84334-4-RR (same clone as 84334-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84334-4-RR (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84334-4-RR (same clone as 84334-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (84334-4-RR, Clone: 241657A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84334-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84334-4-RR against Human TSG101 were performed. The affinity constant is below 1 pM.

TSG101 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84334-4-PBS

Clone No.241657A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

241657A1, ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10

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Product Information

84334-4-PBS targets TSG101 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag28569

  Product name: Recombinant human TSG101 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 214-390 aa of BC002487

  Sequence: GPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTILYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY

• Full Name: tumor susceptibility gene 101
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC002487
• Gene Symbol: TSG101
• Gene ID (NCBI): 7251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q99816
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.