Various lysates were subjected to SDS PAGE followed by western blot with RMX00026 (TSG101 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00026 (TSG101 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using RMX00026
IP result of anti-TSG101 (IP:RMX00026, 4ug; Detection:RMX00026 1:10000) with HeLa cells lysate 1480 ug.
IP result of anti-TSG101 (IP:RMX00026, 4ug; Detection:RMX00026 1:10000) with HeLa cells lysate 1480 ug.
IHC staining of human colon cancer using RMX00026
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00026 (TSG101 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00026 (TSG101 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using RMX00026
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (RMX00026) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (RMX00026) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
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HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected in
HeLa cells
Positive IHC detected in
human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00026 targets TSG101 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00026 (TSG101 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using RMX00026
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00026 (TSG101 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using RMX00026
IP result of anti-TSG101 (IP:RMX00026, 4ug; Detection:RMX00026 1:10000) with HeLa cells lysate 1480 ug.
IF/ICC Figures
IF Staining of HeLa using RMX00026
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (RMX00026) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
TSG101 Multi-Recombinant antibody
Catalog Number
RMX00026
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:300-1:1200 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
