TSNAX Polyclonal antibody

TSNAX Polyclonal Antibody for IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11040-1-AP

Synonyms

translin associated factor X, Translin associated protein X, TRAX, TSNAX

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Validation Data Gallery

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  IP experiment of HeLa using 11040-1-AP

  IP result of anti-TSNAX (IP:11040-1-AP, 4ug; Detection:11040-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IHC staining of human gliomas using 11040-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11040-1-AP (TSNAX antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 11040-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TSNAX antibody 11040-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11040-1-AP targets TSNAX in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSNAX fusion protein Ag1507
• Full Name: translin-associated factor X
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC011797
• Gene Symbol: TSNAX
• Gene ID (NCBI): 7257
• RRID: AB_2303821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.