Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17730-1-AP targets TSNAXIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12130 Product name: Recombinant human TSNAXIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 308-658 aa of BC111018 Sequence: SELQEIQRTSTPRPDWTKCKDVVAGGPERWQMLAEGKNSDQLVDVLLEEIGSGLLREKDFFPGLGYGEAIPAFLRFDGLVENKKPSKKDVVNLLKDAWKERLAEEQKETFPDFFFNFLEHRFGPSDAMAWAYTIFENIKIFHSNEVMSQFYAVLMGKRSENVYVTQKETVAQLLKEMTNADSQNEGLLTMEQFNTVLKSTFPLKTEEQIQELMEAGGWHPSSSNADLLNYRSLFMEDEEGQSEPFVQKLWEQYMDEKDEYLQQLKQELGIELHEEVTLPKLRGGLMTIDPSLDKQTVNTYMSQAFQLPESEMPEEGDEKEEAVVEILQTALERLQVIDIRRVGPREPEPAS Predict reactive species |
| Full Name | translin-associated factor X interacting protein 1 |
| Calculated Molecular Weight | 658 aa, 77 kDa |
| Observed Molecular Weight | 77 kDa, 55 kDa, 42 kDa |
| GenBank Accession Number | BC111018 |
| Gene Symbol | TSNAXIP1 |
| Gene ID (NCBI) | 55815 |
| RRID | AB_2878430 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2TAA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSNAXIP1, also named as TXI1, plays a role in spermatogenesis. It has some isoforms with MW 77 kDa, 55-58 kDa and 42 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TSNAXIP1 antibody 17730-1-AP | Download protocol |
| WB protocol for TSNAXIP1 antibody 17730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TSNAXIP1 Polyclonal antibody (Cat# 17730-1-AP) has 1 total citation. It appears in the journal Andrology (Impact Factor 3.4), published in January 2023. The cited study investigated testis-specific proteins TSNAXIP1 and 1700010I14RIK and their roles in sperm motility and male fertility in mice, using Western blot (WB) with knockout mouse validation. The research field is reproductive biology, specifically spermatogenesis and male infertility.
| Species | Application | Title |
|---|---|---|
Andrology Testis-specific proteins, TSNAXIP1 and 1700010I14RIK, are important for sperm motility and male fertility in mice
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