TSPAN10 Polyclonal antibody

• Featured Product
• KD/KO Validated

TSPAN10 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14430-1-AP

Synonyms

Tspan-10, Tetraspanin-10, Oculospanin, OCSP

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Validation Data Gallery

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  WB analysis of hTERT-RPE1 using 14430-1-AP

  WB result of TSPAN10 antibody (14430-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSPAN10 transfected hTERT-RPE1 cells.

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  IF Staining of hTERT-RPE1 cells using 14430-1-AP

  IF result (nuclear and the base of cilia stain) of anti-TSPAN10 (14430-1-AP, 1:50) with human hTERT-RPE1 (MeOH fixation) cells by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: hTERT-RPE1 cells
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14430-1-AP targets TSPAN10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN10 fusion protein Ag5807
• Full Name: tetraspanin 10
• Calculated Molecular Weight: 36.5 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC032802
• Gene Symbol: TSPAN10
• Gene ID (NCBI): 83882
• RRID: AB_2878057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN10 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN10 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN14, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).