TSPAN12 Polyclonal antibody

TSPAN12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12812-1-AP

Synonyms

NET 2, NET2, Tetraspan NET 2, tetraspanin 12, TM4SF12, Tspan 12, TSPAN12

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Validation Data Gallery

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  WB analysis of HepG2 using 12812-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12812-1-AP (TSPAN12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12812-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12812-1-AP (TSPAN12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12812-1-AP targets TSPAN12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN12 fusion protein Ag3514
• Full Name: tetraspanin 12
• Calculated Molecular Weight: 305 aa, 35 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC031265
• Gene Symbol: TSPAN12
• Gene ID (NCBI): 23554
• RRID: AB_2208667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN12 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN12 regulates retinal vascular development by promoting Norrin/beta-catenin signaling (PMID: 19837033 ). Mutations in TSPAN12 are a relatively frequent cause of familial exudative vitreoretinopathy (FEVR) (PMID: 20159111).