TSPAN13 Polyclonal antibody

• Featured Product
• KD/KO Validated

TSPAN13 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 13570-1-AP

Synonyms

Tspan-13, Transmembrane 4 superfamily member 13, TM4SF13, Tetraspanin-13, Tetraspan NET-6

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Validation Data Gallery

• 

  WB analysis of mouse lung using 13570-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13570-1-AP (TSPAN13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 13570-1-AP

  IP result of anti-TSPAN13 (IP:13570-1-AP, 3ug; Detection:13570-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

13570-1-AP targets TSPAN13 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN13 fusion protein Ag4464
• Full Name: tetraspanin 13
• Calculated Molecular Weight: 204 aa, 22 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC033863
• Gene Symbol: TSPAN13
• Gene ID (NCBI): 27075
• RRID: AB_10597858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN13 (also known as NET6 or TM4SF13) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. Overexpression of TSPAN13 has been reported in prostate cancer, suggesting that TSPAN13 may have an important role in the progression of prostate cancer (PMID: 19148481). TSPAN13 has a calcular molecular weight of 22 kDa. This polyclonal antibody is raised against 100-204aa of human TSPAN13. The experimentally determined molecular mass of 28-35 kDa is larger than the calculated molecular mass, which could be a result of post-translational modifications (PMID: 23648579).

Publications

Species	Application	Title
human	WB	Bosn J Basic Med Sci; Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC).; Authors - Peng Li ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jerome (Verified Customer) (07-08-2020)

Sign background, unclear if specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

TSPAN13 Antibody Western Blot, validation (1:1000 dilution) in HEK293T (Cat no:13570-1-AP)