TSPAN14 Recombinant monoclonal antibody

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• TSPAN14 Recombinant monoclonal antibody (87131-2-RR)

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  WB analysis using 87131-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87131-2-RR (TSPAN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

TSPAN14 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87131-2-RR

Clone No.251354F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DC-TM4F2, Tetraspanin-14, TM4SF14, Transmembrane 4 superfamily member 14, Tspan-14

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Tested Applications

• Positive WB detected in: Caco-2 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87131-2-RR targets TSPAN14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tetraspanin 14
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31-33 kDa
• GenBank Accession Number: BC002920
• Gene Symbol: TSPAN14
• Gene ID (NCBI): 81619
• RRID: AB_3745361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NG11
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN14 is a membrane protein of the tetraspanin superfamily, which is characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN14 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).