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TSPAN15 Polyclonal antibody

TSPAN15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10460-1-AP

Synonyms

2700063A19Rik, NET 7, NET7, Tetraspan NET 7, tetraspanin 15, TM4SF15, Tspan 15, TSPAN15

Validation Data Gallery

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  • Western Blot (WB) analysis of human ovary cancer cells using TSPAN15 Polyclonal antibody (10460-1-AP)

    WB analysis of human ovary cancer using 10460-1-AP

    human ovary cancer were subjected to SDS PAGE followed by western blot with 10460-1-AP (TSPAN15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman ovary cancer

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10460-1-AP targets TSPAN15 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN15 fusion protein Ag0710
Full Name tetraspanin 15
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC003157
Gene Symbol TSPAN15
Gene ID (NCBI) 23555
RRIDAB_2303826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN15 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN15 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

Protocols

Product Specific Protocols
WB protocol for TSPAN15 antibody 10460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Liver Int

Tspan15-ADAM10 signalling enhances cancer stem cell-like properties and induces chemoresistance via Notch1 activation in ICC

Authors - Arihito Yoshizumi
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Maria (Verified Customer) (03-15-2022)

Some unspecific bands in the upper part of the gel but not so much at the expected molecular weight. I used 1:500 solution loading 30ug of protein, but I think it might be possible to use 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y cells
FH

Boyan (Verified Customer) (10-04-2021)

calculated size at 33 and 23 kd. This antibody recognized a very weak band at 23 kd.

  • Applications: Western Blot