TSPAN33 Polyclonal antibody

TSPAN33 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14329-1-AP

Synonyms

hPen, PEN, Penumbra, Proerythroblast new membrane, tetraspanin 33, Tspan 33, TSPAN33

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Validation Data Gallery

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  WB analysis of human brain using 14329-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14329-1-AP (TSPAN33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 14329-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14329-1-AP (TSPAN33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 14329-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 14329-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, human plasma tissue
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14329-1-AP targets TSPAN33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN33 fusion protein Ag5597
• Full Name: tetraspanin 33
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 64-72 kDa
• GenBank Accession Number: BC044244
• Gene Symbol: TSPAN33
• Gene ID (NCBI): 340348
• RRID: AB_10644331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN33 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN33 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN15, and TSPAN17. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

Publications

Species	Application	Title
mouse	WB	J Immunol; The Tetraspanin TSPAN33 Controls TLR-Triggered Macrophage Activation through Modulation of NOTCH Signaling.; Authors - Almudena Ruiz-García