TSPAN6 Polyclonal antibody

TSPAN6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12293-1-AP

Synonyms

A15 homolog, T245, T245 protein, tetraspanin 6, Tetraspanin TM4 D, TM4SF6, TSPAN 6, TSPAN6

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Validation Data Gallery

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  WB analysis of mouse colon using 12293-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12293-1-AP (TSPAN6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12293-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12293-1-AP (TSPAN6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse colon tissue, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12293-1-AP targets TSPAN6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN6 fusion protein Ag2964
• Full Name: tetraspanin 6
• Calculated Molecular Weight: 245 aa, 28 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC012389
• Gene Symbol: TSPAN6
• Gene ID (NCBI): 7105
• RRID: AB_2213446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN6 (also known as TM4SF6) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN6 gene is widely expressed, with high mRNA levels in liver, pancreas, kidney, and ovary (PMID: 9782095). TSPAN6 has been reported as a negative regulator of the RLR pathway by interacting with MAVS in a ubiquitination-dependent manner (PMID: 22908223).