TSPAN7 Polyclonal antibody

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• TSPAN7 Polyclonal antibody (18695-1-AP)

TSPAN7 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 18695-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

TALLA-1, Tetraspanin-7, TALLA 1, MXS1, MRX58

• 

  WB analysis of K-562 using 18695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 18695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 18695-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18695-1-AP (TSPAN7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of K-562 using 18695-1-AP

  IP result of anti-TSPAN7 (IP:18695-1-AP, 4ug; Detection:18695-1-AP 1:500) with K-562 cells lysate 3600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 18695-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18695-1-AP (TSPAN7 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 18695-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18695-1-AP (TSPAN7 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 18695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18695-1-AP (TSPAN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 18695-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18695-1-AP (TSPAN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse pancreas tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18695-1-AP targets TSPAN7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tetraspanin 7
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC018036
• Gene Symbol: TSPAN7
• Gene ID (NCBI): 7102
• RRID: AB_2213450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41732
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN7, also named as A15, MXS1, CD231, MRX58, CCG-B7, TM4SF2, TALLA-1, TM4SF2b and DXS1692E, is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. TSPAN7 is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. TSPAN7 may be involved in cell proliferation and cell motility. It is not solely expressed in T cells, but also expressed in acute myelocytic leukemia cells of some patients. The antibody only recognize the protein TSPAN7, can't recognize the protein TSPAN6.

Publications

What published studies show

The TSPAN7 Polyclonal antibody (Cat# 18695-1-AP) has 12 citations published in journals including Nature Communications, Advanced Science, Journal of Experimental & Clinical Cancer Research, Frontiers in Immunology, Biochemical Pharmacology, Molecular Metabolism, Investigative Ophthalmology & Visual Science, Biology Direct, Annals of Medicine, Frontiers in Oncology, and Frontiers in Genetics. Research fields span cerebral amyloid angiopathy, hepatocellular carcinoma, atherosclerosis, glioma immunotherapy, pancreatic cancer, metabolic health, corneal stem cells, pan-cancer, renal cell carcinoma, bladder cancer, and breast cancer metastasis.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	J Exp Clin Cancer Res; CD151-enriched migrasomes mediate hepatocellular carcinoma invasion by conditioning cancer cells and promoting angiogenesis; Authors - Kangnan Zhang
mouse	WB	Adv Sci (Weinh); CD74 Blockade Disrupts Endothelial Migrasome Signaling to Prevent Inflammatory Macrophage Differentiation and Inhibit Atherosclerotic Progression; Authors - Kangnan Zhang
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	IF	Front Immunol; Functional characterization of TSPAN7 as a novel indicator for immunotherapy in glioma; Authors - Long Chen
		Biochem Pharmacol; TSPAN7 inhibits pancreatic cancer progression by suppressing DNA double-strand break repair and enhances sensitivity to immunochemotherapy; Authors - Hai Lin