TSPAN9 Polyclonal antibody

TSPAN9 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21983-1-AP

Synonyms

NET 5, NET5, PP1057, Tetraspan NET 5, tetraspanin 9, Tspan 9, TSPAN9

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Validation Data Gallery

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  WB analysis of mouse peripheral blood leukocyte using 21983-1-AP

  mouse peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21983-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 21983-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 21983-1-AP

  IP result of anti-TSPAN9 (IP:21983-1-AP, 4ug; Detection:21983-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

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Tested Applications

• Positive WB detected in: mouse peripheral blood leukocyte, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• FC: See 1 publications below

Product Information

The immunogen of 21983-1-AP is TSPAN9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN9 fusion protein Ag17048
• Full Name: tetraspanin 9
• Calculated Molecular Weight: 239 aa, 27 kDa
• Observed Molecular Weight: 29-33 kDa
• GenBank Accession Number: BC071881
• Gene Symbol: TSPAN9
• Gene ID (NCBI): 10867
• RRID: AB_2878960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN9 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN9 is relatively highly expressed in the megakaryocyte/platelet lineage and has a role in regulating platelet function in concert with other platelet tetraspanins and their associated proteins. TSPAN9 has been identified as a glycoprotein (PMID: 18795891). This polyclonal antibody detects endogenous TSPAN9 at a band of 29-33 kDa, which is larger than the calculated molecular weight of TSPAN9 (27 kDa), probably due to glycosylation.

Publications

Species	Application	Title
human,mouse,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li