Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15444-1-AP targets TSPO/PBR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7475 Product name: Recombinant human TSPO protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-169 aa of BC001110 Sequence: MAPPWVPAMGFTLAPSLGCFVGSRFVHGEGLRWYAGLQKPSWHPPHWVLGPVWGTLYSAMGYGSYLVWKELGGFTEKAVVPLGLYTGQLALNWAWPPIFFGARQMGWALVDLLLVSGAAAATTVAWYQVSPLAARLLYPYLAWLAFATTLNYCVWRDNHGWHGGRRLPE Predict reactive species |
| Full Name | translocator protein (18kDa) |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC001110 |
| Gene Symbol | TSPO |
| Gene ID (NCBI) | 706 |
| RRID | AB_3085462 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30536 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TSPO/PBR antibody 15444-1-AP | Download protocol |
| WB protocol for TSPO/PBR antibody 15444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TSPO/PBR Polyclonal antibody (Cat# 15444-1-AP) has a total of 4 citations. These appear in Environmental Research, Scientific Reports, Neurochemical Research, and Cryobiology. The associated research fields include environmental toxicology and microglial neuroinflammation, spinal cord injury and mitochondrial biomarkers, cerebral ischemia-reperfusion injury with autophagy, and oxidative stress/apoptosis in testicular cryopreservation.
| Species | Application | Title |
|---|---|---|
Environ Res Effects of PFOA and emerging alternatives at environmental concentrations on murine- and human-derived microglia: A comparative study. | ||
Sci Rep Identification of a mitochondrial biomarker signature linking neuroinflammation to neuronal dysfunction in spinal cord injury. | ||
Neurochem Res The lncRNA NEAT1 Mediates Neuronal Cell Autophagy and Related Protein Expression After Cerebral Ischemia‒Reperfusion Injury | ||
Cryobiology Procyanidin B2 alleviates damage to mouse testicular tissue after freezing by inhibiting oxidative stress and apoptosis |
