TSPY3 Polyclonal antibody

TSPY3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22257-1-AP

Synonyms

Cancer/testis antigen 78, CT78, TSPY, TSPY1, TSPY3

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Validation Data Gallery

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  WB analysis of human testis using 22257-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22257-1-AP (TSPY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 22257-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 22257-1-AP (TSPY3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22257-1-AP (TSPY3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of rat testis using 22257-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22257-1-AP (TSPY3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 22257-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22257-1-AP (TSPY3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 22257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22257-1-AP (TSPY3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human testis tissue, LNCaP cells
• Positive IHC detected in: human testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22257-1-AP targets TSPY3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPY3 fusion protein Ag17762
• Full Name: testis specific protein, Y-linked 3
• Calculated Molecular Weight: 308 aa, 35 kDa
• Observed Molecular Weight: 39-40 kDa
• GenBank Accession Number: BC075016
• Gene Symbol: TSPY3
• Gene ID (NCBI): 728137
• RRID: AB_11182707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.