Tested Applications
| Positive WB detected in | mouse lung tissue, mouse heart tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13932-1-AP targets TSPYL1 in WB, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4961 Product name: Recombinant human TSPYL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC048969 Sequence: MSGLDGVKRTTPLQTHSIIISDQVPSDQDAHQYLRLRDQSEATQVMAEPGEGGSETVALPPPPPSEEGGVPQDAAGRGGTPQIRVVGGRGHVAIKAGQEEGQPPAEGLAAASVVMAADRSLKKGVQGGEKALEICGAQRSASELTAGAEAEAEEVKTGKCATVSAAVAERESAEVVVKEGLAEKEVMEEQMEVEEQPPEGEEIEVAEEDRLEEEAREEEGPWPLHEALRMDPLEAIQLELDTVNAQADRAFQQLEHKFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQLSAMIRGQDAEMLRYITNLEVKELRHPRTGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRVV Predict reactive species |
| Full Name | TSPY-like 1 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC048969 |
| Gene Symbol | TSPYL1 |
| Gene ID (NCBI) | 7259 |
| RRID | AB_2877991 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0U9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPYL1 antibody 13932-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (catalog no. 13932-1-AP) has 1 citation. It appears in Advanced Science (2024), a study examining TSPYL1 as a regulator of TGFβ signaling through repression of TGFBR1 and TSPYL2. The research field relates to epigenetics and TGFβ signaling pathway regulation in human cells, with the antibody applied in ChIP experiments.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) TSPYL1 as a Critical Regulator of TGFβ Signaling through Repression of TGFBR1 and TSPYL2 |
Reviews
What customers say
One reviewer rated this antibody 4 out of 5 stars for Western Blot using HEK293T cells at a 1:1000 dilution. They noted that while the antibody produces several bands, it clearly detects a band at the expected molecular weight, indicating good specificity for the target despite some additional non-specific signals.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anna (Verified Customer) (02-05-2026) | Produces several bands but does have a clear band at the correct MW.
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