TSPYL5 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• TSPYL5 Polyclonal antibody (27617-1-AP)

• 

  WB analysis using 27617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27617-1-AP (TSPYL5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

TSPYL5 Polyclonal Antibody for WB, ELISA

Cat No. 27617-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KIAA1750, TSPY like 5, TSPY like protein 5

---

Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27617-1-AP targets TSPYL5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26314

  Product name: Recombinant human TSPYL5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-210 aa of BC045630

  Sequence: MSGRSRGRKSSRAKNRGKGRAKARVRPAPDDAPRDPDPSQYQSLGEDTQAAQVQAGAGWGGLEAAASAQLLRLGEEAACRLPLDCGLALRARAAGDHGQAAARPGPGKAASLSERLAADTVFVGTAGTVGRPKNAPRVGNRRGPAGKKAPETCSTAGRGPQVIAGGRQKKGAAGENTSVSAGEEKKEERDAGSGPPATEGSMDTLENVQL

• Full Name: TSPY-like 5
• Calculated Molecular Weight: 417 aa, 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC045630
• Gene Symbol: TSPYL5
• Gene ID (NCBI): 85453
• RRID: AB_3742244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VY4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPYL5 (Testis-specific Y-encoded-like protein 5) is involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. It is also involved in the regulation of p53/TP53, and suppresses p53/TP53 protein levels and promotes its ubiquitination; Its function is dependent on USP7 and independent on MDM2 (PMID: 20079711; 21170034).