TSR2 Polyclonal antibody

TSR2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16263-1-AP

Synonyms

DT1P1A10, TSR2, WGG1

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Validation Data Gallery

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  WB analysis of HeLa using 16263-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16263-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human epididymis using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16263-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16263-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TSR2 antibody 16263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, mouse kidney tissue
• Positive IHC detected in: human liver tissue, human epididymis tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

16263-1-AP targets TSR2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSR2 fusion protein Ag9328
• Full Name: TSR2, 20S rRNA accumulation, homolog (S. cerevisiae)
• Calculated Molecular Weight: 191 aa, 21 kDa
• Observed Molecular Weight: 24-30 kDa
• GenBank Accession Number: BC011825
• Gene Symbol: TSR2
• Gene ID (NCBI): 90121
• RRID: AB_2211381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Acta Histochem; Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers.; Authors - Ekaterina S Snigirevskaya
human	IHC	Cell Biol Int; Ultrastructural traits of apoptosis; Authors - Ekaterina S Snigirevskaya