TSSC1 Polyclonal antibody

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• TSSC1 Polyclonal antibody (19852-1-AP)

TSSC1 Polyclonal Antibody for WB, ELISA

Cat No. 19852-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EARP and GARP complex-interacting protein 1, EIPR1, Endosome-associated recycling protein-interacting protein, Golgi-associated retrograde protein-interacting protein, Protein TSSC1

• 

  WB analysis using 19852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19852-1-AP (TSSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 19852-1-AP

  WB result of TSSC1 antibody (19852-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSSC1 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19852-1-AP targets TSSC1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13923

  Product name: Recombinant human TSSC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-387 aa of BC002485

  Sequence: MEDDAPVIYGLEFQARALTPQTAETDAIRFLVGTQSLKYDNQIHIIDFDDENNIINKNVLLHQAGEIWHISASPADRGVLTTCYNRTSDSKVLTCAAVWRMPKELESGSHESPDDSSSTAQTLELLCHLDNTAHGNMACVVWEPMGDGKKIISLADNHILLWDLQESSSQAVLASSASLEGKGQLKFTSGRWSPHHNCTQVATANDTTLRGWDTRSMSQIYCIENAHGQLVRDLDFNPNKQYYLASCGDDCKVKFWDTRNVTEPVKTLEEHSHWVWNVRYNHSHDQLVLTGSSDSRVILSNMVSISSEPFGHLVDDDDISDQEDHRSEEKSKEPLQDNVIATYEEHEDSVYAVDWSSADPWLFASLSYDGRLVINRVPRALKYHILL

• Full Name: tumor suppressing subtransferable candidate 1
• Calculated Molecular Weight: 387 aa, 44 kDa
• Observed Molecular Weight: ~50 kDa
• GenBank Accession Number: BC002485
• Gene Symbol: TSSC1
• Gene ID (NCBI): 7260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53HC9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSSC1 (Tumor Suppressing Subtransferable Candidate 1), now officially designated EIPR1, encodes a 387-amino-acid WD40 repeat protein that localizes to chromosome 2p25.3 in humans. Although originally identified within a putative tumor-suppressor region at 11p15.5, later genomic mapping reassigned the gene to chromosome 2. Functionally, TSSC1 is a component of the endosomal retrieval machinery, interacting with both the EARP and GARP tethering complexes to regulate protein transport from early endosomes to the trans-Golgi network and recycling endosomes. Gershlick et al. demonstrated that TSSC1 is required for efficient recruitment of GARP to the trans-Golgi network and functions as a regulator of both GARP and EARP in endosomal retrieval pathways (PMID: 27440922).