TSSK3 Polyclonal antibody

TSSK3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12940-1-AP

Synonyms

SPOGA3, STK22C, STK22D, testis serine kinase 3, Testis specific kinase 3, TSK 3, TSSK 3, TSSK3

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Validation Data Gallery

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  WB analysis of mouse testis using 12940-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12940-1-AP (TSSK3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12940-1-AP

  IP result of anti-TSSK3 (IP:12940-1-AP, 4ug; Detection:12940-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human liver cancer using 12940-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12940-1-AP (TSSK3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 12940-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12940-1-AP (TSSK3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12940-1-AP targets TSSK3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSSK3 fusion protein Ag4009
• Full Name: testis-specific serine kinase 3
• Calculated Molecular Weight: 268 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC035354
• Gene Symbol: TSSK3
• Gene ID (NCBI): 81629
• RRID: AB_2877894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Andrology; Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility; Authors - Kaori Nozawa ; KO Validated
mouse	WB	Andrology; CUB domains are not required for OVCH2 function in sperm maturation in the mouse epididymis; Authors - Katarzyna Kent