TST Monoclonal antibody, PBS Only

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  WB analysis using 66018-1-Ig (same clone as 66018-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66018-1-Ig (same clone as 66018-1-PBS)

  WB result of TST antibody (66018-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TST transfected HEK-293 cells. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66018-1-Ig (same clone as 66018-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66018-1-Ig (same clone as 66018-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 66018-1-Ig (same clone as 66018-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66018-1-Ig (same clone as 66018-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 66018-1-Ig (same clone as 66018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66018-1-Ig (same clone as 66018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66018-1-Ig (same clone as 66018-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TST antibody (66018-1-Ig, Clone: 2A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66018-1-PBS

Clone No.2A6C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2A6C5, EC:2.8.1.1, RDS, Rhodanese

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Product Information

66018-1-PBS targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9235

  Product name: Recombinant human TST protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-297 aa of BC010148

  Sequence: MVHQVLYRALVSTKWLAESIRTGKLGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDIEECRDTASPYEMMLPSEAGFAEYVGRLGISNHTHVVVYDGEHLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTSEPSRPEPAVFKATLDRSLLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGLDSGHIRGAVNMPFMDFLTEDGFEKGPEELRALFQTKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSWSEWFRRAPPESRVSQGKSEKA

• Full Name: thiosulfate sulfurtransferase (rhodanese)
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010148
• Gene Symbol: TST
• Gene ID (NCBI): 7263
• RRID: AB_11045660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16762
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.