TTC1 Polyclonal antibody

TTC1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11676-1-AP

Synonyms

TPR repeat protein 1, TPR1, TTC1

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Validation Data Gallery

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  WB analysis of HEK-293 using 11676-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11676-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11676-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11676-1-AP (TTC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11676-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11676-1-AP (TTC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11676-1-AP

  Immunofluorescent analysis of HepG2 cells using 11676-1-AP (TTC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11676-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TTC1 antibody (11676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Product Information

11676-1-AP targets TTC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC1 fusion protein Ag2277
• Full Name: tetratricopeptide repeat domain 1
• Calculated Molecular Weight: 33.5 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000942
• Gene Symbol: TTC1
• Gene ID (NCBI): 7265
• RRID: AB_11182936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar ; KD Validated