TTC19 Polyclonal antibody

TTC19 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20875-1-AP

Synonyms

2010204O13Rik, TPR repeat protein 19, TTC19

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Validation Data Gallery

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  WB analysis using 20875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20875-1-AP (TTC19 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20875-1-AP (TTC19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, rat heart tissue
• Positive IHC detected in: human breast cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

20875-1-AP targets TTC19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC19 fusion protein Ag14974
• Full Name: tetratricopeptide repeat domain 19
• Calculated Molecular Weight: 501 aa, 56 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC011698
• Gene Symbol: TTC19
• Gene ID (NCBI): 54902
• RRID: AB_2878755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTC19 is a subunit of mitochondrial respiratory chain complex III and is proposed to preserve the structural and functional integrity of complex III. Loss-of-function mutations in TTC19 have been found in patients with complex III deficiency associated with heterogeneous neurological syndromes. The precursor TTC19 was detected as a 43 kDa cytoplasmic protein, while the mature TTC19 with the mitochondrial targeting sequence cleaved was observed as 35 kDa by SDS-PAGE. (PMID: 21278747)

Publications

Species	Application	Title
human	WB,IHC	Int J Biol Sci; XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis Failure; Authors - Huijuan Dai ; KD Validated
human	IHC	Elife; Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes; Authors - Aojia Zhuang