Tested Applications
| Positive WB detected in | mouse heart tissue, HeLa cells, rat heart tissue |
| Positive IHC detected in | human breast cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20875-1-AP targets TTC19 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14974 Product name: Recombinant human TTC19 protein Source: e coli.-derived, PKG Tag: GST Domain: 41-383 aa of BC011698 Sequence: EVQVPPSRVAPHGRGPGLLPLLAALAWFSRPAAAEEEEQQGADGAAAEDGADEAEAEIIQLLKRAKLSIMKDEPEEAELILHDALRLAYQTDNKKAITYTYDLMANLAFIRGQLENAEQLFKATMSYLLGGGMKQEDNAIIEISLKLASIYAAQNRQEFAVAGYEFCISTLEEKIEREKELAEDIMSVEEKANTHLLLGMCLDACARYLLFSKQPSQAQRMYEKALQISEEIQGERHPQTIVLMSDLATTLDAQGRFDEAYIYMQRASDLARQINHPELHMVLSNLAAVLMHRERYTQAKEIYQEALKQAKLKKDEISVQHIREELAELSKKSRPLTNSVKL Predict reactive species |
| Full Name | tetratricopeptide repeat domain 19 |
| Calculated Molecular Weight | 501 aa, 56 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC011698 |
| Gene Symbol | TTC19 |
| Gene ID (NCBI) | 54902 |
| RRID | AB_2878755 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6DKK2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TTC19 is a subunit of mitochondrial respiratory chain complex III and is proposed to preserve the structural and functional integrity of complex III. Loss-of-function mutations in TTC19 have been found in patients with complex III deficiency associated with heterogeneous neurological syndromes. The precursor TTC19 was detected as a 43 kDa cytoplasmic protein, while the mature TTC19 with the mitochondrial targeting sequence cleaved was observed as 35 kDa by SDS-PAGE. (PMID: 21278747)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TTC19 antibody 20875-1-AP | Download protocol |
| IHC protocol for TTC19 antibody 20875-1-AP | Download protocol |
| WB protocol for TTC19 antibody 20875-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TTC19 Polyclonal antibody (Cat# 20875-1-AP) has 2 citations. One appears in the International Journal of Biological Sciences (2023), studying XPOT disruption and its role in suppressing triple-negative breast cancer growth through tRNA nuclear exportation and TTC19 expression. The other is published in eLife (2023), examining proteomic signatures and risks of T1 colorectal cancer metastasis to lymph nodes. Both studies utilized the antibody in human samples via Western blot and immunohistochemistry, spanning oncology and cancer biology research fields.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis Failure
| ||
Elife Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes |
Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer reported that the antibody works well for Western Blot in HEK293T cells, indicating reliable performance for this application. Overall, the feedback is positive, confirming the reagent's effectiveness for WB use in this cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
YU (Verified Customer) (01-08-2026) | It works well for WB in HEK293T cell
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