TTC28 Polyclonal antibody, PBS Only

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• TTC28 Polyclonal antibody, PBS Only (25701-1-PBS)

• 

  WB analysis using 25701-1-AP (same clone as 25701-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 25701-1-AP (same clone as 25701-1-PBS)

  WB result of TTC28 antibody (25701-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC28 transfected HeLa cells. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25701-1-AP (same clone as 25701-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 25701-1-AP (same clone as 25701-1-PBS)

  IP result of anti-TTC28 (IP:25701-1-AP, 4ug; Detection:25701-1-AP 1:2500) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 25701-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TTC28 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 25701-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio, TPRBK

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Product Information

25701-1-PBS targets TTC28 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22465

  Product name: Recombinant human TTC28 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 906-1247 aa of BC016465

  Sequence: TGESDQSSTETDSTVKSQEESNPKLDPQELAQKILEETQSHLIAVERLQRSGGQVSKSNNPEDGVQAPSSTAVFRASETSAFSRPVLSHQKSQPSPVTVKPKPPARSSSLPKVSSGYSSPTTSEMSIKDSPSQHSGRPSPGCDSQTSQLDQPLFKLKYPSSPYSAHISKSPRNMSPSSGHQSPAGSAPSPALSYSSAGSARSSPADAPDIDKLKMAAIDEKVQAVHNLKMFWQSTPQHSTGPMKIFRGAPGTMTSKRDVLSLLNLSPRHNKKEEGVDKLELKELSLQQHDGAPPKAPPNGHWRTETTSLGSLPLPAGPPATAPARPLRLPSGNGYKFLSPGR

• Full Name: tetratricopeptide repeat domain 28
• Calculated Molecular Weight: 2481 aa, 271 kDa
• Observed Molecular Weight: 240-271 kDa
• GenBank Accession Number: BC016465
• Gene Symbol: TTC28
• Gene ID (NCBI): 23331
• RRID: AB_3085817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96AY4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression.