TTC35 Polyclonal antibody

• Featured Product
• KD/KO Validated

TTC35 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 25443-1-AP

Synonyms

EMC2, KIAA0103, TPR repeat protein 35, TTC35

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Validation Data Gallery

• 

  WB analysis of Jurkat using 25443-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25443-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 25443-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25443-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 25443-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 25443-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25443-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25443-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 25443-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 25443-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25443-1-AP (TTC35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 25443-1-AP

  IP result of anti-TTC35 (IP:25443-1-AP, 3ug; Detection:25443-1-AP 1:600) with mouse heart tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse heart tissue, K-562 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, human placenta tissue, PC-3 cells, SKOV-3 cells
• Positive IP detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

25443-1-AP targets TTC35 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC35 fusion protein Ag22196
• Full Name: tetratricopeptide repeat domain 35
• Calculated Molecular Weight: 297 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC021667
• Gene Symbol: TTC35
• Gene ID (NCBI): 9694
• RRID: AB_2750836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPR repeat protein 35 (TTC35), also named as tetratricopeptide repeat domain 35, is a 297 amino acid protein, which contains three TPR repeats and belongs to the EMC2 family. TTC35 localizes in the nucleus and cytoplasm. TTC35 is a component of the ER membrane protein complex and may be a putative O-linked glycosyl transferase.

Publications

Species	Application	Title
human	WB	Cell; EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.; Authors - Patrick J Chitwood
human	WB	Mol Cell; WNK1 is an assembly factor for the human ER membrane protein complex.; Authors - Tino Pleiner
human	WB	EMBO Mol Med; SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.; Authors - Alexandre Janer
human	WB	J Cell Biol; A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER; Authors - Tino Pleiner
human	WB	Elife; The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.; Authors - Ashley M Ngo
human	WB	Elife; Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.; Authors - Lakshmi E Miller-Vedam ; KO Validated