TTC4 Polyclonal antibody

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• TTC4 Polyclonal antibody (11878-1-AP)

TTC4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11878-1-AP Copied!

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TPR repeat protein 4, TTC4

• 

  WB analysis of HeLa using 11878-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11878-1-AP

  WB result of TTC4 antibody (11878-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC4 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11878-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11878-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 11878-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11878-1-AP (TTC4 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11878-1-AP targets TTC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2474

  Product name: Recombinant human TTC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-387 aa of BC001276

  Sequence: MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMTRAPSEIDPRENPDLACLQSIIFDEERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDVTAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANLKEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLFLYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVLQHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR

• Full Name: tetratricopeptide repeat domain 4
• Calculated Molecular Weight: 387 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC001276
• Gene Symbol: TTC4
• Gene ID (NCBI): 7268
• RRID: AB_2211700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95801
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

The single review for this product (rated 4/5) describes use in Western Blot on K562 cells at a 1:1000 dilution. The antibody detected the target at the expected molecular weight and was validated by knockdown, confirming specificity. However, the reviewer noted an additional non-specific band appearing below the target band. Overall, the antibody performed well for confirming target expression but showed some background signal.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Coffee (Verified Customer) (03-20-2022)

Verified by knockdown at expected size, but there is an additional non-specific band underneath it.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562 cell

TTC4 Antibody Western Blot validation (1:1000 dilution) in K562 cell (Cat no:11878-1-AP)