TTC5 Polyclonal antibody

TTC5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26112-1-AP

Synonyms

Stress-responsive activator of p300, Strap, Protein Strap

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 26112-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26112-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 26112-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells, RAW 264.7 cells
• Positive IHC detected in: human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26112-1-AP targets TTC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC5 fusion protein Ag23523
• Full Name: tetratricopeptide repeat domain 5
• Observed Molecular Weight: 45 kDa~49 kDa
• GenBank Accession Number: BC053538
• Gene Symbol: TTC5
• Gene ID (NCBI): 91875
• RRID: AB_2880384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.