TTC5 Polyclonal antibody

TTC5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26112-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Protein Strap, Strap, Stress-responsive activator of p300

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of Jurkat using 26112-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 26112-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of RAW 264.7 using 26112-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 26112-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 26112-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 26112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 26112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 26112-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, RAW 264.7 cells
Positive IHC detected in	human colon cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26112-1-AP targets TTC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23523 Product name: Recombinant human TTC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC053538 Sequence: MMADEEEEVKPILQKLQELVDQLYSFRDCYFETHSVEDAGRKQQDVRKEMEKTLQQMEEVVGSVQGKAQVLMLTGKALNVTPDYSPKAEELLSKAVKLEPELVEAWNQLGEVYWKKGDVAAAHTCFSGALTHCRNKVSLQNLSMVLRQLRTDTEDEHSHHVMDSVRQAKLAVQMDVHDGRSWYILGNSYLSLYFSTGQNPKISQQALSAYAQAEKVDRKASSNPDLHLNRATLHKYEESYGEALEGFSRAAALDPAWPEPRQREQQLLEFLDRLTSLLESKGKVKTKKLQSMLGSLRPAHLGPCSDGHYQSASGQKVTLELKPLSTLQPGVNSGAVILGKVVFSLTTE Predict reactive species
Full Name	tetratricopeptide repeat domain 5
Observed Molecular Weight	45 kDa~49 kDa
GenBank Accession Number	BC053538
Gene Symbol	TTC5
Gene ID (NCBI)	91875
RRID	AB_2880384
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N0Z6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TTC5 antibody 26112-1-AP	Download protocol
WB protocol for TTC5 antibody 26112-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TTC5 Polyclonal antibody (Cat# 26112-1-AP) has 1 citation to date. It appears in Cell Communication and Signaling (2025), a study investigating how CARM1 regulates tubulin autoregulation through PI3KC2α R175 methylation. The research field encompasses protein methylation, epigenetic regulation, and cell signaling pathways in human samples, with the antibody applied via Western blot.