TTC7A Polyclonal antibody

TTC7A Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21600-1-AP

Synonyms

TPR repeat protein 7A, KIAA1140

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Validation Data Gallery

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  WB analysis of K-562 using 21600-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 21600-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse small intestine using 21600-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of PC-3 using 21600-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21600-1-AP (TTC7A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 21600-1-AP

  IP result of anti-TTC7A (IP:21600-1-AP, 4ug; Detection:21600-1-AP 1:300) with K-562 cells lysate 4000ug.

• 

  IHC staining of mouse small intestine using 21600-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21600-1-AP (TTC7A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, PC-3 cells, mouse small intestine tissue, mouse colon tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

21600-1-AP targets TTC7A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TTC7A fusion protein Ag16212
• Full Name: tetratricopeptide repeat domain 7A
• Calculated Molecular Weight: 858 aa, 96 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: BC114365
• Gene Symbol: TTC7A
• Gene ID (NCBI): 57217
• RRID: AB_10733221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTC7A (TPR repeat protein 7A) is expressed in enterocytes within the duodenum, ileum, and colon, and has a role in enterocyte survival and function. Mutations in the TTC7A gene can result in a spectrum of intestinal disease, including multiple intestinal atresia (MIA) and very early onset inflammatory bowel diseases (VEOIBD). Functional analysis revealed that TTC7A binds to and facilitates the transport of PI4KIIIa from the trans-Golgi to the plasma membrane, while loss of TTC7A results in dysregulation of PI4KIIIa signaling. This antibody specially recognizes endogenous TTC7A. (24417819)

Publications

Species	Application	Title
human	IHC,IF	Gastroenterology; Mutations in tetratricopeptide repeat domain 7A result in a severe form of very early onset inflammatory bowel disease.; Authors - Yaron Avitzur
human	WB,IHC	Front Immunol; A Novel Homozygous TTC7A Missense Mutation Results in Familial Multiple Intestinal Atresia and Combined Immunodeficiency.; Authors - Wenjun Mou
human	IHC	J Clin Immunol; Bialellic Mutations in Tetratricopeptide Repeat Domain 7A (TTC7A) Cause Common Variable Immunodeficiency-Like Phenotype with Enteropathy.; Authors - Dylan Lawless