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  • KD/KO Validated

TTL Polyclonal antibody

TTL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13618-1-AP

Synonyms

TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase



Tested Applications

Positive WB detected inHeLa cells, mouse brain, rat brain
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13618-1-AP targets TTL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTL fusion protein Ag4526
Full Name tubulin tyrosine ligase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC036819
Gene symbol TTL
Gene ID (NCBI) 150465
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Protocols

Product Specific Protocols
WB protocol for TTL antibody 13618-1-APDownload protocol
IHC protocol for TTL antibody 13618-1-APDownload protocol
IF protocol for TTL antibody 13618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

MARK4 controls ischaemic heart failure through microtubule detyrosination.

Authors - Xian Yu
humanWB

Nature

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanWB

Science

Vasohibins encode tubulin detyrosinating activity.

Authors - Joppe Nieuwenhuis
humanWB

Science

Mitosis. Microtubule detyrosination guides chromosomes during mitosis.

Authors - Marin Barisic
humanWB

Nat Med

Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.

Authors - Christina Yingxian Chen
ratWB

Circ Res

Depletion of Vasohibin 1 Speeds Contraction and Relaxation in Failing Human Cardiomyocytes.

Authors - Christina Yingxian Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (05-11-2022)

Multiple bands corresponding to different isoforms.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse Heart