|Positive WB detected in||HeLa cells, mouse brain, rat brain|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13618-1-AP targets TTL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TTL fusion protein Ag4526|
|Full Name||tubulin tyrosine ligase|
|Calculated molecular weight||377 aa, 43 kDa|
|Observed molecular weight||43-45 kDa|
|GenBank accession number||BC036819|
|Gene ID (NCBI)||150465|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.
MARK4 controls ischaemic heart failure through microtubule detyrosination.
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
Vasohibins encode tubulin detyrosinating activity.
Mitosis. Microtubule detyrosination guides chromosomes during mitosis.
Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.
Depletion of Vasohibin 1 Speeds Contraction and Relaxation in Failing Human Cardiomyocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (05-11-2022)
Multiple bands corresponding to different isoforms.