TTL Monoclonal antibody
TTL Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2E5F8
Cat no : 66076-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat brain tissue, fetal human brain tissue, mouse brain tissue, pig brain tissue, SH-SY5Y cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 66076-1-Ig is TTL Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TTL fusion protein Ag4708 |
Full Name | tubulin tyrosine ligase |
Calculated molecular weight | 377 aa, 43 kDa |
Observed molecular weight | 43 kDa |
GenBank accession number | BC036819 |
Gene symbol | TTL |
Gene ID (NCBI) | 150465 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.
Protocols
Product Specific Protocols | |
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WB protocol for TTL antibody 66076-1-Ig | Download protocol |
IHC protocol for TTL antibody 66076-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Carcinogenesis Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing. | ||
Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
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