Tested Applications
| Positive WB detected in | LNCaP cells, fetal human brain tissue, SH-SY5Y cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66076-1-Ig targets TTL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4708 Product name: Recombinant human TTL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-377 aa of BC036819 Sequence: MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIQPPISNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGILISSEASELLDFIDNQGQVHVIQKYLEHPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLTSALNITLESSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDVEQPQTQPAAFIKL Predict reactive species |
| Full Name | tubulin tyrosine ligase |
| Calculated Molecular Weight | 377 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC036819 |
| Gene Symbol | TTL |
| Gene ID (NCBI) | 150465 |
| RRID | AB_11182924 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NG68 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TTL antibody 66076-1-Ig | Download protocol |
| WB protocol for TTL antibody 66076-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TTL Monoclonal antibody (2E5F8), catalog number 66076-1-Ig, has 8 citations published in Nature Communications, Cell Reports, Journal of Molecular Cell Biology, Journal of Cell Science, Carcinogenesis, IUBMB Life, Methods in Cell Biology, and bioRxiv. Research fields include microtubule dynamics, tubulin tyrosination/detyrosination, kinetochore function, axonal transport, trophoblast biology, genome editing, DNA repair, and neuroinflammation.
| Species | Application | Title |
|---|---|---|
Nat Commun α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments
| ||
Cell Rep Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.
| ||
J Cell Sci Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination | ||
Carcinogenesis Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing. | ||
IUBMB Life Tyrosine and Phenylalanine Activate Neuronal DNA Repair but Exhibit Opposing Effects on Global Transcription and Adult Female Mice Are Resilient to TyrRS/YARS1 Depletion. |
