TTL Monoclonal antibody

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  WB analysis using 66076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 66076-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66076-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

TTL Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66076-1-Ig

Clone No.2E5F8

Publications(7)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Synonyms

2E5F8, EC:6.3.2.25, Tubulin tyrosine ligase, tubulin tyrosine ligase, Tubulin--tyrosine ligase

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Tested Applications

• Positive WB detected in: LNCaP cells, fetal human brain tissue, SH-SY5Y cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

66076-1-Ig targets TTL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TTL fusion protein Ag4708
• Full Name: tubulin tyrosine ligase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC036819
• Gene Symbol: TTL
• Gene ID (NCBI): 150465
• RRID: AB_11182924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NG68
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Publications

Species	Application	Title
human	IF	J Mol Cell Biol; Tubulin detyrosination promotes human trophoblast syncytium formation.; Authors - Rui Wang
human		Carcinogenesis; Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing.; Authors - Amrendra Mishra
		Methods Cell Biol; Dissecting the role of the tubulin code in mitosis.; Authors - Luísa T Ferreira
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated
mouse	IF	J Cell Sci; Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination; Authors - Anja Konietzny
human	WB	Nat Commun; α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments; Authors - Hugo Girão ; KO Validated