TTL Monoclonal antibody
TTL Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
human, mouse, rat, pig
WB, IHC, IF, ELISA
Cat no : 66076-1-Ig
Validation Data Gallery
|Positive WB detected in||rat brain tissue, fetal human brain tissue, mouse brain tissue, pig brain tissue, SH-SY5Y cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 1 publications below|
|IF||See 1 publications below|
The immunogen of 66076-1-Ig is TTL Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||TTL fusion protein Ag4708|
|Full Name||tubulin tyrosine ligase|
|Calculated molecular weight||377 aa, 43 kDa|
|Observed molecular weight||43 kDa|
|GenBank accession number||BC036819|
|Gene ID (NCBI)||150465|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.
|Product Specific Protocols|
|WB protocol for TTL antibody 66076-1-Ig||Download protocol|
|IHC protocol for TTL antibody 66076-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing.
Methods Cell Biol
Dissecting the role of the tubulin code in mitosis.
J Mol Cell Biol
Tubulin detyrosination promotes human trophoblast syncytium formation.
Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.