TTL Monoclonal antibody

TTL Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2E5F8

Cat no : 66076-1-Ig

Synonyms

TTL, Tubulin tyrosine ligase, tubulin tyrosine ligase

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Validation Data Gallery

• 

  WB analysis of rat brain using 66076-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66076-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66076-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66076-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66076-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat brain tissue, fetal human brain tissue, mouse brain tissue, pig brain tissue, SH-SY5Y cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 66076-1-Ig is TTL Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TTL fusion protein Ag4708
• Full Name: tubulin tyrosine ligase
• Calculated molecular weight: 377 aa, 43 kDa
• Observed molecular weight: 43 kDa
• GenBank accession number: BC036819
• Gene symbol: TTL
• Gene ID (NCBI): 150465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Publications

Species	Application	Title
human		Carcinogenesis; Generation of focal mutations and large genomic deletions in the pancreas using inducible in vivo genome editing.; Authors - Amrendra Mishra
		Methods Cell Biol; Dissecting the role of the tubulin code in mitosis.; Authors - Luísa T Ferreira
human	IF	J Mol Cell Biol; Tubulin detyrosination promotes human trophoblast syncytium formation.; Authors - Rui Wang
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated