TTL Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 82763-5-RR (same clone as 82763-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82763-5-RR (TTL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82763-5-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82763-5-RR (same clone as 82763-5-PBS)

  WB result of TTL antibody (82763-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells. This data was developed using the same antibody clone with 82763-5-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TTL Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 82763-5-PBS

Clone No.3P9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 3P9

---

Product Information

82763-5-PBS targets TTL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4526

  Product name: Recombinant human TTL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-377 aa of BC036819

  Sequence: MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIQPPISNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGILISSEASELLDFIDNQGQVHVIQKYLEHPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLTSALNITLESSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDVEQPQTQPAAFIKL

• Full Name: tubulin tyrosine ligase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC036819
• Gene Symbol: TTL
• Gene ID (NCBI): 150465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NG68
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.