Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21447-1-AP targets TTLL12 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13804 Product name: Recombinant human TTLL12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 293-644 aa of BC001070 Sequence: DINPVVHPHGHIFKVYTDVQQVASSLTHPRFTLTQSEADADILFNFSHFKDYRKLSQERPGVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQFVSYFQQRERWGEDNHWICKPWNLARSLDTHVTKSLHSIIRHRESTPKVVSKYIESPVLFLREDVGKVKFDIRYIVLLRSVRPLRLFVYDVFWLRFSNRAFALNDLDDYEKHFTVMNYDPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQVACAKPPPLGLCDYPSSRAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYHPTFFNDVFSTLFLDQPGGCHVTCLV Predict reactive species |
| Full Name | tubulin tyrosine ligase-like family, member 12 |
| Calculated Molecular Weight | 644 aa, 74 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC001070 |
| Gene Symbol | TTLL12 |
| Gene ID (NCBI) | 23170 |
| RRID | AB_3669368 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14166 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TTLL12 antibody 21447-1-AP | Download protocol |
| IHC protocol for TTLL12 antibody 21447-1-AP | Download protocol |
| WB protocol for TTLL12 antibody 21447-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TTLL12 Polyclonal antibody (Cat# 21447-1-AP) has 1 total citation. It was published in International Journal of Clinical and Experimental Pathol (Int J Clin Exp Pathol) in 2020. The cited study investigated TTLL12 expression in ovarian cancer and its correlation with poor patient outcomes, placing this antibody's use in the fields of ovarian cancer research and clinical pathology.
| Species | Application | Title |
|---|---|---|
Int J Clin Exp Pathol TTLL12 expression in ovarian cancer correlates with a poor outcome. |
