TTPA Monoclonal antibody

TTPA Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3E7D10

Cat no : 67431-1-Ig

Synonyms

Alpha TTP, alphaTTP, ATTP, AVED, TPP1, TTP1, TTPA

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Validation Data Gallery

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  WB analysis of rat liver using 67431-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 67431-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 67431-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67431-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67431-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 67431-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 67431-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat liver tissue, pig liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67431-1-Ig targets TTPA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TTPA fusion protein Ag13587
• Full Name: tocopherol (alpha) transfer protein
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC058000
• Gene Symbol: TTPA
• Gene ID (NCBI): 7274
• RRID: AB_2882669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.