TTYH1 Polyclonal antibody

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  WB analysis using 26973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 26973-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 26973-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TTYH1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26973-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

hTTY1, Protein tweety homolog 1, TTYH1, tweety homolog 1 (Drosophila)

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human gliomas; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26973-1-AP targets TTYH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25290

  Product name: Recombinant human TTYH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 264-390 aa of BC011347

  Sequence: EAATAVGLSDFCSNPDPYVLNLTQEETGLSSDILSYYLLCNRAVSNPFQQRLTLSQRALANIHSQLLGLEREAVPQFPSAQKPLLSLEETLNVTEGNFHQLVALLHCRSLHKDYGAALRGLCEDALE

• Full Name: tweety homolog 1 (Drosophila)
• Calculated Molecular Weight: 49-50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC011347
• Gene Symbol: TTYH1
• Gene ID (NCBI): 57348
• RRID: AB_2880707
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H313
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TTYH1, protein tweety homolog 1, is a glycosylated protein composed of five predicted transmembrane segments, which belongs to the tweety family. The expression of TTYH1 is restricted mainly to neural tissue and is up-regulated in astrocytoma, glioma and several other cancers. TTYH1 has some isoforms with MW of 49-50 kDa, 35 kDa and 26 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	J Cancer; Elevated expression of Tweety homologue 3 predicts poor clinical outcomes in ovarian cancer.; Authors - Junhan Zhou
mouse	IF	Nat Commun; Endolysosomal processing of neuron-derived signaling lipids regulates autophagy and lipid droplet degradation in astrocytes; Authors - Jagannatham Naidu Bhupana