TUB Polyclonal antibody

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  WB analysis of mouse brain using 17928-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 17928-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 17928-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17928-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17928-1-AP

  IP result of anti-TUB (IP:17928-1-AP, 4ug; Detection:17928-1-AP 1:1000) with mouse brain tissue lysate 3680ug.

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  IHC staining of human brain using 17928-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17928-1-AP (TUB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17928-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17928-1-AP (TUB antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

TUB Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17928-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Synonyms

rd5, TUB, tubby homolog (mouse), Tubby protein homolog

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

17928-1-AP targets TUB in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12354

  Product name: Recombinant human TUB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 212-561 aa of BC075032

  Sequence: GQSDHAQDAGETAAGGGERPSGQDLRATMQRKGISSSMSFDEDEEDEEENSSSSSQLNSNTRPSSATSRKSVREAASAPSPTAPEQPVDVEVQDLEEFALRPAPQGITIKCRITRDKKGMDRGMYPTYFLHLDREDGKKVFLLAGRKRKKSKTSNYLISVDPTDLSRGGDSYIGKLRSNLMGTKFTVYDNGVNPQKASSSTLESGTLRQELAAVCYETNVLGFKGPRKMSVIVPGMNMVHERVSIRPRNEHETLLARWQNKNTESIIELQNKTPVWNDDTQSYVLNFHGRVTQASVKNFQIIHGNDPDYIVMQFGRVAEDVFTMDYNYPLCALQAFAIALSSFDSKLACE

• Full Name: tubby homolog (mouse)
• Calculated Molecular Weight: 561 aa, 62 kDa
• Observed Molecular Weight: 56 kDa, 62-67 kDa
• GenBank Accession Number: BC075032
• Gene Symbol: TUB
• Gene ID (NCBI): 7275
• RRID: AB_2212236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50607
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cells; Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.; Authors - Julie J Hong
human	ChIP,WB	Cell Rep; NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.; Authors - Yuetong Wang ; KD Validated
mouse	IF	Front Neurosci; Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti; Authors - Laura A Lindner