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  • KD/KO Validated

TUB Polyclonal antibody

TUB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 17928-1-AP

Synonyms

rd5, TUB, tubby homolog (mouse), Tubby protein homolog



Tested Applications

Positive WB detected inmouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17928-1-AP targets TUB in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUB fusion protein Ag12354
Full Name tubby homolog (mouse)
Calculated Molecular Weight 561 aa, 62 kDa
Observed Molecular Weight 56 kDa, 62-67 kDa
GenBank Accession NumberBC075032
Gene Symbol TUB
Gene ID (NCBI) 7275
RRIDAB_2212236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TUB antibody 17928-1-APDownload protocol
IHC protocol for TUB antibody 17928-1-APDownload protocol
IP protocol for TUB antibody 17928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cells

Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.

Authors - Julie J Hong
humanChIP,WB

Cell Rep

NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.

Authors - Yuetong Wang
  • KD Validated
mouseIF

Front Neurosci

Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti

Authors - Laura A Lindner