Tested Applications
| Positive WB detected in | mouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17928-1-AP targets TUB in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12354 Product name: Recombinant human TUB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 212-561 aa of BC075032 Sequence: GQSDHAQDAGETAAGGGERPSGQDLRATMQRKGISSSMSFDEDEEDEEENSSSSSQLNSNTRPSSATSRKSVREAASAPSPTAPEQPVDVEVQDLEEFALRPAPQGITIKCRITRDKKGMDRGMYPTYFLHLDREDGKKVFLLAGRKRKKSKTSNYLISVDPTDLSRGGDSYIGKLRSNLMGTKFTVYDNGVNPQKASSSTLESGTLRQELAAVCYETNVLGFKGPRKMSVIVPGMNMVHERVSIRPRNEHETLLARWQNKNTESIIELQNKTPVWNDDTQSYVLNFHGRVTQASVKNFQIIHGNDPDYIVMQFGRVAEDVFTMDYNYPLCALQAFAIALSSFDSKLACE Predict reactive species |
| Full Name | tubby homolog (mouse) |
| Calculated Molecular Weight | 561 aa, 62 kDa |
| Observed Molecular Weight | 56 kDa, 62-67 kDa |
| GenBank Accession Number | BC075032 |
| Gene Symbol | TUB |
| Gene ID (NCBI) | 7275 |
| RRID | AB_2212236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50607 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TUB antibody 17928-1-AP | Download protocol |
| IHC protocol for TUB antibody 17928-1-AP | Download protocol |
| IP protocol for TUB antibody 17928-1-AP | Download protocol |
| WB protocol for TUB antibody 17928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Tubby (TUB) antibody (Cat# 17928-1-AP) has 6 total citations published in Neuron, Science Translational Medicine, Cell Communication and Signaling, Molecular Cells, Cell Reports, and Frontiers in Neuroscience. Research fields include outer hair cell stereocilia structure, leptin sensitivity and obesity, adipocyte differentiation, ciliary formation and trafficking, T-cell-mediated tumor immunity, and ciliary protein distribution in the organ of Corti.
| Species | Application | Title |
|---|---|---|
Sci Transl Med The TUB variant impairs leptin sensitivity and AgRP neuronal response, leading to obesity | ||
Cell Commun Signal miR-10b-5p regulates adipocyte lineage commitment and adipogenesis via targeting of Gata6 and Tubby. | ||
Mol Cells Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking. | ||
Cell Rep NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.
| ||
Front Neurosci Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti |
