TUBB3-specific/TUJ1 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 80128-3-RR (same clone as 80128-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80128-3-RR (TUBB3-specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using TUBB3-specific antibody (80128-3-RR, Clone: 250851G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP00550-2

  The mean TUBB3 concentration was determined to be 6.4 ng/mL in SH-SY5Y cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP00550-2

  Cytometric bead array standard curve of MP00550-2, TUBB3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80128-3-PBS. Detection antibody: 80128-2-PBS. Standard: SY01676. Range: 0.039-2.5 ng/mL.

TUBB3-specific/TUJ1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 80128-3-PBS

Clone No.250851G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

beta tubulin, Tubulin beta 3 chain, Tubulin beta III, TUBB3, TUBB4

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Product Information

80128-3-PBS targets TUBB3-specific/TUJ1 as part of a matched antibody pair:

MP00550-2: 80128-3-PBS capture and 80128-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: tubulin, beta 3
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_001197181
• Gene Symbol: TUBB3
• Gene ID (NCBI): 10381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13509
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.