TUFT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TUFT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23385-1-AP

Synonyms

TUFT1, Tuftelin, tuftelin 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 23385-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23385-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23385-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23385-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 23385-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23385-1-AP (TUFT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 23385-1-AP is TUFT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TUFT1 fusion protein Ag20020
• Full Name: tuftelin 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC002933
• Gene Symbol: TUFT1
• Gene ID (NCBI): 7286
• RRID: AB_2879270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Pathol Oncol Res; Tuftelin 1 (TUFT1) Promotes the Proliferation and Migration of Renal Cell Carcinoma via PI3K/AKT Signaling Pathway.; Authors - Hua Lin ; KD Validated