TUFT1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• TUFT1 Polyclonal antibody (23385-1-AP)

• Featured Product
• KD/KO Validated

TUFT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23385-1-AP Copied!

Publications (2)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

tuftelin1, tuftelin 1, Tuftelin

• 

  WB analysis of HEK-293 using 23385-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 23385-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 23385-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 23385-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23385-1-AP (TUFT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 23385-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23385-1-AP (TUFT1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 23385-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23385-1-AP (TUFT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for TUFT1 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23385-1-AP targets TUFT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20020

  Product name: Recombinant human TUFT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-365 aa of BC002933

  Sequence: MNGTRNWCTLVDVHPEDQAAAGRKTYAMVSSHSAGHSLASELVESHDGHEEIIKVYLKGRSGDKMIHEKNINQLKSEVQYIQEARNCLQKLREDISSKLDRNLGDSLHRQEIQVVLEKPNGFSQSPTALYSSPPEVDTCINEDVESLRKTVQDLLAKLQEAKRQHQSDCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGMETEHQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHLDDMLKSQQRKVRQMIEQLQNSKAVIQSKDATIQELKEKIAYLEAENLEMHDRMEHLIEKQISHGNFSTQARAKTENPGSIRISKPPSPKPMPVIRVVET

• Full Name: tuftelin 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC002933
• Gene Symbol: TUFT1
• Gene ID (NCBI): 7286
• RRID: AB_2879270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9NNX1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TUFT1 Polyclonal antibody (Cat# 23385-1-AP) has 2 citations. These appear in Cell Death & Disease and Pathology - Oncology Research. The research fields cover cancer progression, specifically TUFT1's role in suppressing centrosome amplification and mitotic spindle multipolarity, as well as its promotion of renal cell carcinoma proliferation and migration via the PI3K/AKT signaling pathway. Both studies utilized Western blotting for validation.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cell Death Dis; TUFT1 regulates cancer progression by suppressing centrosome amplification and mitotic spindle multipolarity; Authors - Shaojie Feng
human	WB	Pathol Oncol Res; Tuftelin 1 (TUFT1) Promotes the Proliferation and Migration of Renal Cell Carcinoma via PI3K/AKT Signaling Pathway.; Authors - Hua Lin ; KD Validated