TWF2 Polyclonal antibody

TWF2 Polyclonal Antibody for WB, ELISA

Cat No. 14769-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

A6r, A6-related protein, A6RP, Protein tyrosine kinase 9-like, PTK9L

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 14769-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14769-1-AP (TWF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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Tested Applications

Positive WB detected in

A431 cells, HEK-293 cells, HepG2 cells, L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14769-1-AP targets TWF2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6553 Product name: Recombinant human TWF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC000327 Sequence: MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASQEPVGRWDQDYDRAVLPLLDAQQPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAFPLQPEAQRALQQLKQKMVNYIQMKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFHLEIAKKIEIGDGAELTAEFLYDEVHPKQHAFKQAFAKPKGPGGKRGHKRLIRGPGENGDDS Predict reactive species
Full Name	twinfilin, actin-binding protein, homolog 2 (Drosophila)
Calculated Molecular Weight	40 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC000327
Gene Symbol	TWF2
Gene ID (NCBI)	11344
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6IBS0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TWF2, also known as PTK9L and A6RP, contains a consensus sequence for actin-depolymerizing proteins and 2 ATP-binding motifs. TWF2 is an actin-binding protein that inhibits actin polymerization by sequestering G-actin. TWF2 plays an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (PMID: 10406962).